| Ignite Christian Academy | Rock Rapids | K-12 | 2,257 | 41.7 | Christian (nondenominational) |
| Ankeny Christian Academy ✓ | Ankeny | PreK-12 | 510 | 14.7 | Christian (nondenominational) |
| Pella Christian Grade School ✓ | Pella | PreK-8 | 489 | 13.8 | Christian (nondenominational) |
| Grand View Christian School ✓ | Des Moines | PreK-5 | 458 | 19.2 | Christian (nondenominational) |
| Souixland Christian School ✓ | Sioux City | PreK-12 | 449 | 7.8 | Christian (nondenominational) |
| Netherlands Reformed Christian School ✓ | Rock Valley | PreK-12 | 422 | 13.6 | Calvinist |
| Trinity Lutheran Church School | Davenport | PreK-8 | 353 | 17.7 | Lutheran Church (Missouri Synod) |
| Unity Christian High School ✓ | Orange City | 9-12 | 327 | 14.5 | Calvinist |
| Orange City Christian School ✓ | Orange City | PreK-8 | 313 | 104.3 | Calvinist |
| Waterloo Christian School ✓ | Waterloo | PreK-12 | 312 | 14.9 | Christian (nondenominational) |
| Pella Christian High School ✓ | Pella | 9-12 | 272 | 12.3 | Calvinist |
| Morning Star Academy ✓ | Bettendorf | PreK-12 | 227 | 12.6 | Christian (nondenominational) |
| Rock Valley Christian School ✓ | Rock Valley | PreK-8 | 202 | 11.3 | Christian (nondenominational) |
| Cedar Valley Christian School ✓ | Cedar Rapids | PreK-12 | 201 | 10.8 | Christian (nondenominational) |
| Joshua Christian Academy ✓ | Des Moines | K-12 | 199 | 8.3 | Christian (nondenominational) |
| Mt Olive Lutheran School ✓ | Des Moines | PreK-8 | 191 | 13.5 | Lutheran Church (Missouri Synod) |
| Oskaloosa Christian School ✓ | Oskaloosa | K-8 | 182 | 20.2 | Christian (nondenominational) |
| Tri-State Christian School ✓ | Asbury | K-12 | 181 | 9.1 | Christian (nondenominational) |
| Heritage Christian School ✓ | North Liberty | PreK-8 | 178 | 12.6 | Christian (nondenominational) |
| St. Paul's Lutheran School ✓ | Waverly | PreK-6 | 148 | 11 | Evangelical Lutheran Church in America |
| Valley Lutheran School ✓ | Cedar Falls | K-12 | 146 | 9.4 | Lutheran Church (Missouri Synod) |
| Clear Lake Classical ✓ | Clear Lake | PreK-12 | 135 | 8.7 | Christian (nondenominational) |
| Hull Protestant Reformed Christian School ✓ | Hull | K-8 | 133 | 12.7 | Christian (nondenominational) |
| Iowa Great Lakes Lutheran School ✓ | Spencer | K-7 | 119 | 13.5 | Lutheran Church (Missouri Synod) |
| Trinity Lutheran School ✓ | Boone | PreK-8 | 116 | 13.6 | Lutheran Church (Missouri Synod) |
| Faith Academy ✓ | Iowa City | K-6 | 115 | 10.4 | Christian (nondenominational) |
| Hillcrest Academy | Kalona | 6-12 | 105 | 7.4 | Mennonite |
| Sanborn Christian School ✓ | Sanborn | PreK-8 | 103 | 8.9 | Christian (nondenominational) |
| Ottumwa Christian School ✓ | Ottumwa | PreK-12 | 97 | 8.4 | Christian (nondenominational) |
| Sheldon Christian School ✓ | Sheldon | PreK-8 | 96 | 8.4 | Christian (nondenominational) |
| North Iowa Christian School ✓ | Mason City | K-12 | 84 | 9.3 | Christian (nondenominational) |
| St. Paul Lutheran School ✓ | Fort Dodge | PreK-8 | 84 | 12 | Lutheran Church (Missouri Synod) |
| Lutheran Interparish School ✓ | Williamsburg | PreK-8 | 83 | 8.5 | Lutheran Church (Missouri Synod) |
| Community Christian School ✓ | Fort Dodge | PreK-8 | 83 | 10.9 | Christian (nondenominational) |
| Clarinda Lutheran School | Clarinda | K-8 | 81 | 9.4 | Lutheran Church (Missouri Synod) |
| Pathway Christian School ✓ | Kalona | PreK-12 | 80 | 8 | Mennonite |
| Zion Lutheran School | Denison | PreK-9 | 73 | 8.8 | Lutheran Church (Missouri Synod) |
| Newton Christian Day School ✓ | Newton | PreK-8 | 70 | 10.3 | Christian (nondenominational) |
| Ireton Christian Schools ✓ | Ireton | PreK-8 | 70 | 9.6 | Christian (nondenominational) |
| Trinity Christian High School ✓ | Hull | 9-12 | 69 | 8.1 | Calvinist |